Investigation of the Antibacterial Activity of three types of medicated soaps on Staphylococcus aureus

Travail d'étude , 2018 , 38 Pages , Note: 3.0

Biologie - Microbiologie, Biologie Moléculaire

Following invasion of the human body by bacteria, most bacteria produce substances that are toxic to the human body. In the fight against these infections, the production of antibacterial substances (synthetic compounds of different forms) has been a major step in solving this problem. In this study, three medicated soaps: Dettol, Pharmapur and Tetmosol were investigated for their antibacterial activity at different concentrations against Staphylococcus aureus using the disc diffusion method. Saline was used as a negative control and a readymade antibiotic impregnated disc (Ciprofloxacin) was used as a positive control. Bacterial identification was by standard microbiological techniques which included: Colonial examination, Gram staining and biochemical testing. Dettol medicated soap had the highest antibacterial activity (26mm at 1/10 soap dilution) while Tetmosol showed the least antibacterial activity (6mm at 1/103 soap dilution). S. aureus was found on 4 of the 6 samples that were analysed giving a prevalence of 66.67%. All the medicated soaps showed antibacterial activity which depended on the concentration (dilution) of soap sample on S. aureus; hence, the use of antibacterial soaps is recommended as a means of reducing risks of transmission and infection by bacteria.

TABLE OF CONTENTS

DEDICATION

ACKNOWLEDGMENTS

ABSTRACT

TABLE OF CONTENTS

LIST OF TABLE AND FIGURES

CHAPTER 1: INTRODUCTION AND LITERATURE REVIEW1
1.1 INTRODUCTION
1.2 LITERATURE REVIEW
1.2.1: The skin and the normal skin microbiota
1.2.2: Burden, prevalence and epidemiology of Staphylococcus aureus infections
1.2.3: Clinical manifestations and pathogenesis of Staphylococcus aureus Infections
1.2.4: Resistance of Staphylococcus aureus to antibacterial agents
1.2.5: Prevention, control and treatment of Staphylococcus aureus infections
1.2.6: Medical relevance of medicated (antibacterial) soap
1.3 STATEMENT OF THE PROBLEM
1.4 HYPOTHESIS
1.5 OBJECTIVES
1.5.1: General objectives
1.5.2: Specific objectives
1.6 LIMITATIONS OF STUDY

CHAPTER 2: MATERIALS AND METHODS
2.1 MATERIALS
2.1.1: Materials and instruments
2.1.2: Preparation of 70% alcohol
2.1.3: Preparation of soap solutions
2.1.4: Preparation of soap impregnated discs
2.1.5: Preparation of McFarland standard
2.1.6: Media preparation.
2.1.6.1: Preparation of Mannitol salt agar
2.1.6.2: Preparation of Muller Hinton agar
2.2 METHODS
2.2.1: Sample collection and isolation of microorganisms
2.2.2: Identification and confirmation of isolates
2.2.3: Antimicrobial susceptibility testing

CHAPTER 3: RESULTS
3.1: Prevalence of Staphylococcus aureus on skin of subjects
3.2: Antimicrobial patterns

CHAPTER 4: DISCUSSION
4.1 Conclusion
4.2 Recommendation

REFERENCES

APPENDIX

APPENDIX

• Université: University of Buea
• Cours: Microbiology
• Note: 3.0
• Auteur: Chikaeze Akaolisa (Auteur)
• Année de publication: 2018
• Pages: 38
• N° de catalogue: V389100
• ISBN (ebook): 9783668629622
• ISBN (Livre): 9783668629639
• Langue: anglais
• mots-clé: Staphylococcus aureus, Medicated soaps, Prevalence, Antibacterial activity investigation, Disc diffusion method