Microbe supported enhanced production of Rosmarinic acid of medicinal plants in vitro

Etude Scientifique , 2017 , 12 Pages

Biologie - Microbiologie, Biologie Moléculaire

In the described study , the bio-enhancement of Rosmarinic acid( RA) was done successfully in suspension culture of Salvia officinalis and Ocimum sanctum suspended with E coli K12 entrapped alginate beads.It is reported that Tyrosin Amino transferase has a key role in the production of Rosmarinic acid , which is present in E coli K 12. Quantification by HPTLC indicated that the maximum RA content was noted in 4 weeks old (2050 mg/l) in Salvia officinalis suspension culture fed with Tyrosin 10 mg/100ml followed by 4 weeks old Ocimum sanctum suspension culture fed with Tyrosin 10 mg/100ml(715 mg/l), that are about 18 fold and 5 folds enhancement as compared to control. The method for significantly enhancement of RA was reported for the first time.

Contents

Introduction

Material and Methods
Plant material
Suspension media

Preparation of beads of E coli K12

Down stream process for Rosmarinic acid extraction

High Performance Thin Layer Chromatography (HPTLC) Analysis

Infra Red Spectral Studies

Statistical analysis

Results and Discussion

Conclusion

Acknowledgement

References

• Auteur: Dr. Pratibha Chaturvedi (Auteur)
• Année de publication: 2017
• Pages: 12
• N° de catalogue: V355224
• ISBN (ebook): 9783668426948
• ISBN (Livre): 9783668426955
• Langue: anglais
• mots-clé: enhanced, rosmarinic, salvia, ocimum, using, hptlc, rosmarinic acid, medical plants, in vitro, salvia officinalis, ocimum sanctum, e coli K 12, alginate beads