The colonial diameter of Lactococcus lactis spp. lactis isolates on MRS agar was promoted by providing negative atmospheric pressure and /or enrichment of negative atmosphere by CO2 gas in vacuum desiccator. In this study, application of negative atmospheric pressure or enrichment of negative atmosphere by CO2 gas at 722 mmHg was observed for maximum (2.5 mm) colony size of L. lactis spp. lactis on agar surface. No significant difference was observed in the colonial diameter in the presence of negative atmospheric pressure or enrichment of negative atmosphere by CO2 gas at 722 to 608 mmHg pressure.
Inhaltsverzeichnis (Table of Contents)
- Abstract
- Key Words
- INTRODUCTION
- MATERIALS AND METHODS
- Samples
- Bacterial strains and growth conditions
- Identification
- Effect of different levels of O2 and CO2 gases on colony formation
- RESULTS AND DISCUSSION
- CONCLUSION
- REFERENCES
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This study aimed to investigate the effect of different O2 and CO2 concentrations on the growth and colony formation of Lactococcus lactis spp. lactis, a beneficial microbe widely used in the dairy and food industry. The research sought to determine if modifying the atmospheric pressure and CO2 levels could enhance the colony size of L. lactis spp. lactis on agar surfaces.
- The impact of negative atmospheric pressure and CO2 enrichment on the growth of L. lactis spp. lactis.
- The optimal levels of O2 and CO2 for maximizing colony size.
- The role of oxygen in the growth and survival of L. lactis spp. lactis.
- Comparison of the effects of negative atmospheric pressure and CO2 enrichment on colony formation.
- The potential applications of these findings for routine cultivation of L. lactis spp. lactis in laboratory settings.
Zusammenfassung der Kapitel (Chapter Summaries)
The introduction provides background information on L. lactis spp. lactis, its importance in the food industry, and its microaerophilic nature. The materials and methods section outlines the experimental procedures, including sample collection, bacterial strains used, identification techniques, and the specific methodology for evaluating the effect of varying O2 and CO2 levels on colony formation. The results and discussion section presents the findings of the study, demonstrating the positive effect of negative atmospheric pressure and CO2 enrichment on colony size. The conclusion summarizes the key findings and their implications for laboratory cultivation of L. lactis spp. lactis.
Schlüsselwörter (Keywords)
The study focuses on Lactococcus lactis spp. lactis, a beneficial microbe in the food industry. The primary research focuses on the impact of O2 and CO2 concentrations, specifically negative atmospheric pressure and CO2 enrichment, on the growth and colony formation of L. lactis spp. lactis. Key terms include microaerophilic, colony size, negative atmospheric pressure, CO2 enrichment, and routine cultivation.
- Citation du texte
- Priti Khemariya (Auteur), Sudhir Singh (Auteur), Gopal Nath (Auteur), Anil K. Gulati (Auteur), 2016, Effect of different levels of O2 and CO2 gases on the growth of Lactococcus lactis spp. lactis, Munich, GRIN Verlag, https://www.grin.com/document/316237
