Micobial degradation of triphenyl methane dyes

Trabajo de Investigación , 2012 , 15 Páginas

Química - Bioquímica

Try phenyl methane dyes have been found in soil and river sediments as consequences of improper chemical waste disposal. 10000 dyes and pigments are produced annually world wide amounting to 7*107tones which are hazardous and pose serious environmental problems. It is estimated that 10-15% of the dye is lost in the effluent during the dying process. Try phenyl methane dye decolorizing bacteria have been isolated; there are few reports of specific enzymes that decolorize these dyes. Isolate bacterial strains which had the capability to decolorize textile dye like bromophenol blue, crystal violet and malacate green. We estimate the decolorization percentage for all the three tri phenyl methane dyes and quantify the activity of the TMR enzyme that degrades try phenyl methane dye and characterize the dye degrading organism as pseudomonas species.

Inhaltsverzeichnis

• Abstract
• Keywords
• Introduction
• Materials and Methods
  • Isolation of the dye degrading organism
  • Decolorization assay
  • TMR Enzyme assay
  • Characterization of the bacterial isolates
• Results and Discussion
• Conclusion
• References

Zielsetzung und Themenschwerpunkte

This research aims to investigate the microbial degradation of triphenyl methane dyes, a significant environmental concern due to their widespread use and persistence in the environment. The study focuses on isolating and characterizing bacterial strains capable of decolorizing these dyes, specifically bromophenol blue, crystal violet, and malachite green. The research also aims to quantify the activity of the TMR enzyme responsible for degrading triphenyl methane dyes and to identify the bacterial species involved in the degradation process.

• Isolation and characterization of dye-degrading bacteria
• Decolorization of triphenyl methane dyes by bacterial strains
• Quantification of TMR enzyme activity
• Identification of the bacterial species responsible for dye degradation
• Environmental implications of microbial dye degradation

Zusammenfassung der Kapitel

The introduction provides a comprehensive overview of the environmental impact of triphenyl methane dyes, highlighting their widespread use, persistence in the environment, and the challenges associated with their removal. The study's objectives and the importance of microbial degradation in addressing this environmental issue are outlined. The materials and methods section details the experimental procedures employed in the study, including the isolation of dye-degrading bacteria, the decolorization assay, the TMR enzyme assay, and the characterization of the bacterial isolates. The results and discussion section presents the findings of the study, including the identification of bacterial strains capable of decolorizing triphenyl methane dyes, the quantification of TMR enzyme activity, and the characterization of the bacterial species involved in the degradation process. The conclusion summarizes the key findings of the study and discusses the implications of the research for environmental remediation and the development of sustainable dye-treatment technologies.

Schlüsselwörter

The key terms and focus themes of the text include triphenyl methane dyes, TMR enzyme, dye degradation, bacterial decolorization, Pseudomonas species, environmental pollution, and sustainable dye treatment technologies. The study investigates the microbial degradation of triphenyl methane dyes, focusing on the isolation, characterization, and activity of dye-degrading bacteria, particularly Pseudomonas species. The research highlights the potential of microbial processes for addressing the environmental challenges posed by dye pollution and contributes to the development of sustainable dye treatment technologies.

• Curso: MASTER DEGREE
• Autor: Prasad Mp (Autor)
• Año de publicación: 2012
• Páginas: 15
• No. de catálogo: V286443
• ISBN (Ebook): 9783656867104
• ISBN (Libro): 9783656867111
• Idioma: Inglés
• Etiqueta: micobial