Neutraceutical, Phytochemical characterization and Antibacterial activity of Medicinal plant Moringa Oleifera

Tesis Doctoral / Disertación , 2012 , 132 Páginas , Calificación: 3

Química - Química analítica

Moringa oleifera, an important medicinal plant is one of the most widely cultivated species of the family Moringaceae. It is highly valued from time immemorial because of its vast medicinal properties. The present study provides all necessary information regarding of four parts such as flower, leaves, seed and pulp of moringa like biochemical, phytochemical, mineral, antibacterial activity and its nutritional value. The benefits of essential nutrients and minerals for maintaining good health were also highlighted in this study.
The results of proximate analysis of Moringa oleifera revealed that the protein (9.37%), carbohydrate (7.33%), ascorbic acid (2.10%) and total soluble sugar (0.73%) were highest in flower as compared to leaves, seed and pulp. While free amino acid (9.84%) was found to be higher in seed, total phenol (0.29%) was higher in leaves and reducing sugar (0.43%) higher in pulp of the moringa. The result of qualitative analysis of amino acid represented that lysine, glycine, threonine, valine, Isoleucine, tryptophan, alanine and cystein were present in moringa. The flower also contained higher amounts of crude fibre (0.23%) as well as moisture (90.56%), while fat (15.53%) content was found higher in seed. The dry matter (30.40%) and total ash (2.12%) content were higher in leaves. The ash content represented minerals in different amounts. The higher amount of potassium was found in flower (50.9%), seed (40.7%) and pulp (77.00%). Leaves contained higher amount of Calcium (57.18%). However Aluminum (10.00%) and Magnesium (6.07%) were found only in leaves. The result of heavy metal (zinc, lead and cadmium) and analysis represented that flower, leaves, seed and pulp have zinc (Zn), lead (Pb) and cadmium (Cd) found in lower amount then permissible limit for human body.
The results of phytochemical analysis showed that terpenoids and steroids were present in all parts of moringa. Alkaloids present only in seed. Flavonoid was present in flower and seed, saponins was present in leaves, and tannin was present in leaves and seed.
The result of antibacterial activity of different types of sample (flower, leaves. seed and pulp) of moringa showed that salmonella typhii was effectively inhibited to all the extracts studied. But Escherichia coli were not inhibited by any extract. Methanolic extract of flower, leaves, seed and pulp were highly sensitive against the salmonella typhii bacteria

Inhaltsverzeichnis (Table of Contents)

• Abbreviation
• Abstract
• CHAPTER I-Introduction
• CHATER II -Review and Literature
  • 2.1 History of MoringaOleifera
  • 2.2 Moringa Species
  • 2.3 Classification MoringaOleifera
  • 2.4 Common Name of Moringa Oleifera
  • 2.5 Morphology and Physical Characteristics
  • 2.6Distribution
  • 2.7 Cultivation
  • 2.8 Chemical Composition
  • 2.9Biochemical Parameter
  • 2.10 Photochemistry
  • 2.11 Antibacterial Activity
  • 2.12 Minerals
  • 2.13 Use of Moringaoleifera
• CHAPTER-III MATERIAL AND METHOD
  • 3.1 Experimental site
  • 3.2 Experimental material
  • 3.3 Glass wares and polywares
  • 3.4 Chemicals and solvents
  • 3.5 Biochemical parameter
  • 3.6 Determination of phytochemicals
  • 3.7 To record qualitative analysis of amino acid by TLC
  • 3.8To assay antibacterial activity of differentextract of moringa
  • 3.9 To find out mineral composition of different parts of moringa using XRF
  • 3.10 Estimation of sodium by flame photometer
  • 3.11 To study heavy metal in different parts of moringa using VA
• CHAPTER-IV RESULT AND DISCUSSION
  • 4.1 Biochemical parameter
  • 4.2 Phytochemicals analysis
  • 4.3 To record qualitative analysis of amino acid by TLC
  • 4.4 To Assay antibacterial activity of different extract of moringa oleifera
  • 4.5To find out mineral composition of different parts of moringa using XRF
  • 4.6 Estimation of sodium by Flame Photometer
  • 4.7 To study heavy metal in different parts of moringa using VA
• CONCLUSION
• REFERENCES
• Fig No. Title
  • LIST OF FIGURES
  • 1.1 Different use of moringa oleifera
  • 2.1 Moringa oleifera
  • 2.2 Trunk and leaves of moringa oleifera
  • 2.3 Use of moringa oleifera
  • 2.4 Treating dirty water with moringa stenopetala
  • 3.1 Fiber therm
  • 3.2 UV spectrophotometer
  • 3.3 Silica gel plate
  • 3.4 Developing TLC plate
  • 3.5 Hydraulic press
  • 3.6 Sample for XRF analysis
  • 4.1 % Dry matter and Moisture in different parts of moringa
  • 4.2 % Ash in different parts of moringa
  • 4.3 % Fibre in different parts of moringa
  • 4.4 % Fat in different parts of moringa
  • 4.5 % Carbohydrate in different parts of moringa
  • 4.6 % Protein in different parts of moringa
  • 4.7 % Soluble sugar in different parts of moringa
  • 4.8 % Reducing Sugar in different parts of moringa
  • 4.9 % Phenol in different parts of moringa

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This research paper aims to comprehensively investigate the neutraceutical and phytochemical properties of the medicinal plant Moringa oleifera, along with its antibacterial activity. The study focuses on various aspects of the plant, including its history, morphology, chemical composition, and potential applications.

• Neutraceutical and Phytochemical Characterization of Moringa oleifera
• Antibacterial Activity of Moringa oleifera Extracts
• Mineral Composition and Heavy Metal Content in Different Parts of Moringa oleifera
• Biochemical Parameters and Qualitative Analysis of Amino Acids
• Potential Applications of Moringa oleifera in Various Fields

Zusammenfassung der Kapitel (Chapter Summaries)

Chapter I: Introduction This chapter provides a general overview of the research topic, highlighting the importance of Moringa oleifera as a medicinal plant and its potential benefits. It introduces the objectives and scope of the study, outlining the key areas of investigation.

Chapter II: Review and Literature This chapter delves into the existing literature on Moringa oleifera, covering its history, species, classification, common names, morphology, distribution, cultivation, chemical composition, biochemical parameters, photochemistry, antibacterial activity, mineral content, and various uses. It provides a comprehensive review of the scientific knowledge surrounding this plant.

Chapter III: Material and Method This chapter details the experimental methodology employed in the study. It describes the experimental site, materials, glassware, chemicals, solvents, and procedures used for biochemical parameter analysis, phytochemical determination, amino acid analysis by TLC, antibacterial activity assays, mineral composition analysis using XRF, sodium estimation by flame photometer, and heavy metal analysis using VA.

Chapter IV: Result and Discussion This chapter presents the findings of the study, analyzing the results obtained from the various experiments conducted. It discusses the biochemical parameters, phytochemical analysis, amino acid profile, antibacterial activity, mineral composition, sodium content, and heavy metal levels in different parts of Moringa oleifera. The chapter also interprets the results and draws conclusions based on the findings.

Schlüsselwörter (Keywords)

The keywords and focus themes of the text include Moringa oleifera, neutraceuticals, phytochemicals, antibacterial activity, medicinal plant, biochemical parameters, mineral composition, heavy metals, amino acids, TLC, XRF, VA, and potential applications.

• Universidad: Kachchh University (Department of Chemistry)
• Curso: MSc
• Calificación: 3
• Autores: Taslimahemad Khatri (Autor), Pragna L. Mori (Autor), Dr. Vijay R. Ram (Autor), Dr. Pragnesh N. Dave (Autor), Dr. Balubhai A. Golakia (Autor)
• Año de publicación: 2012
• Páginas: 132
• No. de catálogo: V279200
• ISBN (Ebook): 9783656723400
• ISBN (Libro): 9783656723370
• Idioma: Inglés
• Etiqueta: neutraceutical, phytochemical, antibacterial, medicinal, moringa, oleifera