Effect of concurrent administration of an antibiotic and an anti-inflammatory drug on the immunotoxicity of bacterial endotoxins

Thèse de Doctorat , 2014 , 114 Pages

Médecine - Technique Biomédicale

P. aeruginosa is a gram-negative bacterium that causes a variety of diseases in compromised hosts. Bacterial endotoxins such as LPS are the major outer surface membrane components present in almost all gram-negative bacteria and act as extremely strong stimulators of innate immunity and inflammation of the airway. The present study was undertaken to determine the effect of combined administration of Gentamicin (GENT) as antibiotic and Dexamethasone (DEXA) as ant-inflammatory drug on some physiological, immunological and histological parameters. After determination of LD50 of P. aeruginosa, mice groups were injected with DEXA, GENT and LPS alone or in combination. LPS single injection caused a significant increase of total protein, globulin, total leukocyte count, lymphocytes, neutrophils and level of IgM and IgG. DEXA induced an increase of serum total lipid, ALT and AST levels, neutrophilia and lymphopenia. GENT administration increased serum total protein, globulin, total lipid, ALT and AST levels. Physiological and immunological examination demonstrated that combined treatment has a significant effect as decreaseing serum total protein, globulin, lymphocytes and IgG level than single treatment. Histological examination demonstrated that the inflammation of thymus, spleen, lymph node and liver decreases in mice received combined treatment than those received individual treatment. Concurrent administration of DEXA and GENT has greatest effect in protecting organs against damage in case of endotoximia.

Inhaltsverzeichnis

• Abstract
• Introduction and Aim of Work
• Review of Literature
• Materials and Methods
• Results
• Discussion
• Summary
• References

Zielsetzung und Themenschwerpunkte

This study investigates the impact of combined administration of Gentamicin (GENT) and Dexamethasone (DEXA) on the immunotoxicity of bacterial endotoxins, specifically LPS from Pseudomonas aeruginosa. The study aims to determine the effects of this combined treatment on various physiological, immunological, and histological parameters in mice. The research focuses on understanding how the combined administration of an antibiotic and an anti-inflammatory drug influences the body's response to bacterial endotoxins.

• The effect of combined administration of Gentamicin and Dexamethasone on the immunotoxicity of bacterial endotoxins.
• The impact of combined treatment on physiological, immunological, and histological parameters in mice.
• The role of Gentamicin and Dexamethasone in modulating the immune response to bacterial endotoxins.
• The potential benefits and risks of combined antibiotic and anti-inflammatory therapy in the context of bacterial endotoxins.

Zusammenfassung der Kapitel

The study begins with an introduction that provides background information on Pseudomonas aeruginosa, bacterial endotoxins, and the role of LPS in inducing immune responses. The introduction also highlights the potential risks associated with excessive exposure to bacterial endotoxins and the importance of understanding the host response to LPS. The study then delves into the mechanisms by which glucocorticoids, such as Dexamethasone, modulate immune reactions and antagonize the effects of bacterial endotoxins. The introduction also discusses the properties and uses of Gentamicin, an aminoglycoside antibiotic with a broad spectrum of activity.

The study then outlines the materials and methods used to conduct the experiment. This section details the experimental design, the animal model used, the administration of the drugs and LPS, and the methods employed to assess physiological, immunological, and histological parameters. The results section presents the findings of the study, including the effects of single and combined treatments on various parameters such as total protein, globulin, total leukocyte count, lymphocytes, neutrophils, IgM, IgG, total lipid, ALT, and AST levels. The results are presented in a clear and concise manner, highlighting the significant findings of the study.

The discussion section analyzes the results of the study, interpreting the findings in the context of previous research and providing insights into the mechanisms underlying the observed effects. The discussion also explores the implications of the findings for the treatment of bacterial endotoxins and the potential benefits and risks of combined antibiotic and anti-inflammatory therapy. Finally, the study concludes with a summary of the key findings and their significance.

Schlüsselwörter

The keywords and focus themes of the text include Pseudomonas aeruginosa, LPS, Dexamethasone, Gentamicin, immunoglobulin, total protein, albumin, globulin, total lipid, AST, ALT, total leukocyte count, immunotoxicity, inflammation, and combined therapy. The study investigates the effects of combined administration of an antibiotic and an anti-inflammatory drug on the immune response to bacterial endotoxins, specifically LPS from Pseudomonas aeruginosa. The research focuses on understanding the impact of this combined treatment on various physiological, immunological, and histological parameters in mice.

• Université: Cairo University (Faculty of Science)
• Cours: Immunology
• Auteur: Azza El Amir (Auteur)
• Année de publication: 2014
• Pages: 114
• N° de catalogue: V276241
• ISBN (ebook): 9783656697619
• ISBN (Livre): 9783656699187
• Langue: anglais
• mots-clé: effect, concurrent, administration, antibiotic, anti-inflammatory, drugs, immunotoxicity, bacterial, endotoxins