Flow Cytometry of Intracellular Calcium in Platelets

Fachbuch , 2012 , 30 Seiten

Medizin - Pharmakologie, Arzneimittelwesen, Pharmazie

During the last years flow cytometry has been developed into an important diagnostic tool with a wide range of applications. Whereas the differentiation of leukocytes is most often encountered, flow cytometry also renders itself especially useful in the study of platelets. Commonly, surface markers are of key interest, but the fact that platelets can be conveniently activated in vitro offers the opportunity to investigate intracellular signal transduction processes, which lead to the release of calcium into the cytosol. And it is this rise of intracellular calcium that flow cytometry is able to detect in real time through the use of suitable calcium-sensitive markers.
It is the aim of this book to put together an overview of this technique and therefore to provide a sound starting ground for its application to clinical and experimental investigations and also for its further development.

Contents

Introduction
The Role of Calcium in Intracellular Signal Transduction
Sources of Calcium
Signal Transduction
Activation of Platelets
Adhesion
Shape Change
Aggregation
Secretion
Procoagulant Activity
Soluble Platelet Agonists
Inhibition of Platelets by cAMP

Flow Cytometry
Principle
Parameters
Monoclonal Antibodies
Isotype Control
Compensation
Monoclonal Antibodies Used for the Flow Cytometric Characterization of Platelets
Intracellular Probes
Presentation and Analysis of Data
Dotplots and Identification of Cells
Further Analysis
Regions and Gates

The Use of Fluo-3 as Intracellular Calcium Indicator
Brief Introduction
Fluo-3

Application
Flow Cytometric Analysis

Conclusion

References

Abbreviations

Index

• Autor: Thomas Gamsjäger (Autor:in)
• Erscheinungsjahr: 2012
• Seiten: 30
• Katalognummer: V190163
• ISBN (eBook): 9783656146520
• ISBN (Buch): 9783656146629
• Sprache: Englisch
• Schlagworte: flow, cytometry, intracellular, calcium, platelets