Chronic Heart Failure (CHF) is combined with various metabolic shifts. The continuous adrenergic stress results in a metabolic shift increasing glycolysis similar to a fetal metabolism. However also insulin resistance (IR) was reported triggering low glucose uptake
and mitochondrial uncoupling and therefore reactive oxygen species (ROS) production reduce cardiac contractility. The adrenergic increased free fatty acids (FFA) are metabolized to ketone bodies (mainly β-hydroxybutyrate (OHB)) which are also energy stocks for brain and heart. Elevated blood ketone levels have been reported during CHF similar to diabetes. Clinically a correlation between ketone body blood level and severity of CHF has been discovered. However it remains unclear whether this is a result of metabolic changes or a compensatory mechanism. The aim of this study was to show, that rat cardiomyocytes (H9c2) treated with Phenylephrine (PE) for hypertrophy are slightly more susceptible to OHB at concentrations similar found in patients with CHF and that OHB reduces cell area significantly.
Table of Contents
Abstract
Zusammenfassung
Introduction
Theoretical Background
Chronic Heart Failure
Mitochondrial Alterations
Metabolic Shift
Insulin Resistance
Ketone Bodies
Oxidative Stress
Microcurrent Therapy
Aim of this experiment
Results
Hypertrophic Model
H9c2
Primary cardiomyocytes (SHR7)
Electro Microcurrent treated primary (SHR7) cells
Cell growth under ß-Hydroxybutyrate treatment
Cell proliferation Assay with alamarBlue®
Hypertrophic growth under ß-Hydroxybutyrate treatment
ROS assay
Immunohistochemical staining (Mitochondrial distribution)
H9c2
SHR7
Discussion
Hypertrophic Model
Mitochondrial Activity Assay
ROS Assay
Immunostaining
Conclusion
References
Eigenständigkeitserklärung
Attachment:
Abbreviations
Materials
Methods
Hypertrophic Model
Hypertrophic Measurement
HE-Staining
MitoTracker®
ROS-Measurement
OHB Proliferation Assay
AlamarBlue®-Assay
Cristal Violet-Assay
Immunostaining (Caveolin-3)
Immunostaining (a-Tubulin)
Microcurrent treatment of SHR7 cells
- Citar trabajo
- BSc Matthias Pilecky (Autor), 2010, The Influence of 3-Hydroxybutyrate and Microcurrent Treatment on Cardiomyocytes during Simulated Hypertrophy, Múnich, GRIN Verlag, https://www.grin.com/document/168718
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