Presentation with the title "Usage of NMR Spectroscopy in Profiling/ metabonomics" and the following contents:
- NMR Spectroscopy;
- Spin-spin coupling;
- Why 1H NMR is adequate for the analysis of fruit juices;
- Usage of Amix viewer for Data Analysis;
- Objectives;
- Preparation of phosphate buffer (pH 6 - 7 at 25°C);
- Sample Preparation;
- Main experiment (noesypr) (nuclear Overhauser effect spectroscopy Presaturation);
- Why the Identification of Metabolites;
- Organic acids occuring in Fruit juices;
- Sugars and amino acids occuring in fruit juices;
- Fruit juices;
- Identification of metabolites in the extrakted apple juice;
- Identification of metabolites in the commercial apple juices;
- Investigation of the vitamine C content;
- Mixing of unripe fruits in apple juices;
- Identification of metabolites in the orange juice;
- Identification of metabolites in the multivitamine juice;
- Sugar content in commertial fruit juices;
- Changes in the fruit juice samples after three weeks;
- Investigation of some Coca Cola brands;
- Sugar free Cola (discount brand);
- Summary
Inhaltsverzeichnis (Table of Contents)
- NMR Spectroscopy
- Definition
- Usage of Amix viewer for Data Analysis
- How to show a series of spectra on top of selected spectrum
- Matching spectra
- Creating a new spectra base (Sbase)
- Displaying spectra in overlaid mode
- Displaying a series of spectra in horizontal- and vertical splitting
- Why 1H NMR is adequate for the analysis of fruit juices
- Usage of Amix viewer for Data Analysis
- Matching the spectra of pure compounds to the compound mixture from the spectra base
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This work explores the application of Nuclear Magnetic Resonance (NMR) spectroscopy in profiling and metabonomics, particularly in the analysis of biological fluids such as fruit juices. It aims to demonstrate the advantages and capabilities of NMR for these purposes.
- Introduction to NMR spectroscopy and its principles
- Applications of NMR in profiling and metabonomics
- Advantages of NMR for analyzing biological fluids
- Data analysis using Amix viewer software
- Specific case studies of fruit juice analysis
Zusammenfassung der Kapitel (Chapter Summaries)
- NMR Spectroscopy: This chapter introduces the fundamental concepts of NMR spectroscopy, including its definition, basic principles, and applications in chemical structure elucidation, characterization, and purity control.
- Why 1H NMR is adequate for the analysis of fruit juices: This chapter explores the specific reasons why NMR spectroscopy is a suitable technique for analyzing fruit juices. It highlights the advantages of NMR, such as non-destructive nature, quantitative and structural information, high throughput, and minimal sample preparation.
- Usage of Amix viewer for Data Analysis: This chapter provides a detailed overview of the Amix viewer software and its capabilities for analyzing NMR data. It covers various features like displaying spectra, matching spectra, creating spectra bases, and other applications relevant to profiling and metabonomics.
Schlüsselwörter (Keywords)
The primary keywords and focus topics include NMR spectroscopy, profiling, metabonomics, fruit juices, biological fluids, Amix viewer, data analysis, non-destructive method, quantitative information, structural information, high throughput, minimal sample preparation, and spectra base.
- Quote paper
- Sadik Mejid (Author), 2016, Usage of NMR Spectroscopy in Profiling Metabonomics, Munich, GRIN Verlag, https://www.grin.com/document/475230
