Cellulose is one the most abundant carbon sources present on the earth. In the book, the author reported on production optimization and characterization of cellulolytic enzymes by thermophilic bacteria. Various adaptation strategies adapted by thermophilic bacteria are also discussed along with.
The thermophilic bacterial strain produced cellulase in the CMC broth, pH 7 containing 0.5% peptone, 0.5% malt extract, 0.2% ammonium sulphate, 0.2% ammonium nitrate and 0.2% NaCl at 50˚C. Interestingly, the bacteria could not grow at 37˚C, confirming its thermophilic nature. Further, the cellulase was characterized after getting partially purified by ammonium sulphate precipitation method. The partially purified cellulase may be employed to hydrolyze agricultural wastes to produce bioethanol, i.e. biofuels. Thus, the present research may help solve issues of crisis of renewable energy as well as environmental pollution.
CONTENTS
Introduction
Work flow
Review of Literature
Materials and Methods
Results
Discussion
Summary
References
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Upload your own papers! Earn money and win an iPhone X.