Isolation, characterization and optimization of dye degrading bacteria from natural source

An overview

Scientific Study , 2016 , 40 Pages , Grade: 1.5

Biology - Micro- and Molecular Biology

In this study an attempt was made to evaluate the colour degradation capabilities by collecting the contaminated soil sample from Kalady area and serial dilution was done upto 10-6. From the dilution 10-5 was taken and spread plated on Nutrient agar. From the above plate, isolated colonies was obtained which was found to be Bacillus sp and Pseudomonas sp respectively by morphological, microscopical and biochemical method. The isolated colonies was taken for degradation studies with 1% dye and 1% inoculum in Nutrient broth and OD values and colour change was noted. It was found to be Bacillus sp has more degrading capacity in yellow colour than Pseudmonas sp. The optimization studies was done with Bacillus sp having different concentration of colour (2, 4, 6) with varying pH (4,6,8) and temperature (37°C, 40°C and room temperature). The result was found to be having the concentration of colour with 4% having pH 4 and temperature 37°C.

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Isolation, characterization and optimization of dye degrading bacteria from natural source: an overview

Abstract

1. Introduction
1.1 Objectives

2. Review of literature
2.1 Reactive dyes
2.2 Removal methods
2.3 Physical methods
2.4 By adsorption
2.5 By ion exchange
2.6 By membrane filtration
2.7 Chemical methods
2.8 Advanced oxidation process (AOP)
2.9 Electrochemical Method
2.10 Biological methods
2.11 Anaerobic treatment
2.12 Enzymatic treatments

3. Hypothesis

4. Materials and Methods
4.1 Study area
4.2 Soil sample collection
4.3 Quantitative analysis of microorganisms present in the collected soil
4.4 Isolation of potential dye decolourizers
4.5 Pure culture preparation
4.6 Morphological and biochemical tests
4.7 Statistical analysis

5. Results and discussion
5.1 Dye degradation studies
5.2 Optimization studies

6. Conclusions

References

• College: Mar Augusthinose College
• Grade: 1.5
• Authors: Dr. Prem Jose Vazhacharickal (Author), John Joseph (Author), Jiby John Mathew (Author), Sajeshkumar N.K. (Author), Delmy Abraham (Author)
• Publication Year: 2016
• Pages: 40
• Catalog Number: V370601
• ISBN (eBook): 9783668478596
• ISBN (Book): 9783668478602
• Language: English
• Tags: Azo dyes, Dye degradation, Biochemical identification